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Part:BBa_K763003:Design

Designed by: Pedro Luis Dorado Morales   Group: iGEM12_Valencia_Biocampus   (2012-09-07)

pGlnA + Gene encoding ZsYellow1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 689
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No design considerations needed.

Source

The sequence of the promoter comes from E. coli genome, whereas the sequence encoding the AmCyan fluorescent protein is not natural, since it was optimized for codon usage and maximum fluorescence production.

References

  1. Reitzer, L.J., Movsas, B. andMagasanik, B. (1989)Activation of glnA transcription by Nitrogen Regulator I (NRI)- phosphate in Escherichia coli: evidence for a long-range physical interaction between NRI-phosphate and RNA polymerase.Journal of bacteriology, 171 (10):5512-5522
  2. Wang, L., Guo, Y. and Gralla, J.D. (1999) Regulation of sigma 54-dependent transcription by core promoter sequences: role of - 12 region nucleotides. Journal of bacteriology, 181 (24):7558–7565
  3. Barrios, H., Valderrama, B. y Morett, E. (1999) Compilation and analysis of sigma54-dependent promoter sequences. Nucleics Acids Research, 27 (22):4305-4313.